ANALISIS POHON FILOGENETIK GEN ITS1 Amanita muscaria DARI BERBAGAI NEGARA

Authors

  • Tri Mutia Andini
  • Afifatul Achyar Universitas Negeri Padang

DOI:

https://doi.org/10.30605/biogenerasi.v10i2.5799

Keywords:

Amanita muscaria, Genetic diversity, Phylogenetic tree

Abstract

The purpose of this study is to determine the genetic diversity of the ITS1 gene from Amanita muscaria originating from various countries and create a phylogenetic tree based on ITS1 gene data to show the evolutionary relationship and distribution history of Amanita muscaria in various countries.The method used in this study is the Neighbor-Joining Method which is used to construct phylogenetic trees quickly and efficiently. Bootstrap tests (10,000 replicates) indicate the confidence level of the tree branches. Evolution was calculated using the Maximum Composite Likelihood method. The analysis involved 13 nucleotide sequences and was performed with MEGA11 software. The results of this study are The phylogenetic tree of Amanita muscaria shows two main clades with bootstrap values of 27 and 39, which reflect the level of confidence in the group of specimens. The first clade with a value of 27 includes specimens AB096048.1, AJ549964.1, AB080778.1, AB081295.1, and LN877747.1, while the second clade with a value of 39 includes AB015700.1, AB081294.1, AB080779.1, and AB080983.1. The branch with a bootstrap value of 63 shows greater genetic diversity. Specimens AB096048.1 and AJ549964.1 and AB080777.1 and AB096052.1 have very close evolutionary relationships.

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References

Ashley, L., Wagner, M., Yan, Z., & Kulka, M. (2023). Amanita muscaria extract potentiates production of proinflammatory cytokines by dsRNA-activated human microglia. Frontiers in Pharmacology. https://doi.org/10.3389/fphar.2023.1102465
Barbare, J. (2022). Amanita sect. Phalloideae: Two interesting non-lethal species from West Africa. Mycological Progress. https://doi.org/10.1007/s11557-022-01778-0
Cao, J., Zhang, Z., Liu, D., Li, F., Cheng, X., Zhang, Y., Wang, H., Chang, Y., & Li, Z. (2009). ITS sequence analysis of 8 Amanita species from Tibetan and Yunnan. Journal of Yunnan University.
Felsenstein J. (1985). Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39:783-791.
Liu, Y., Liu, J.-K., Kumla, J., Suwannarach, N., & Lumyong, S. (2023). Taxonomic novelties and new records of Amanita subgenus Amanitina from Thailand. Journal of Fungi. https://doi.org/10.3390/jof9060601
Milton, T. D., Park, S.-C., Wang, W. Y., Keller, N. P., & Pringle, A. (2023). Pangenomics of the death cap mushroom Amanita phalloides, and of Agaricales, reveals dynamic evolution of toxin genes in an invasive range. The ISME Journal. https://doi.org/10.1038/s41396-023-01432-x
Milton, T., Drott, S.-C., Park, Y., Wang, W., Harrow, L., Keller, N. P., & Pringle, A. (2023). Pangenomics of the death cap mushroom Amanita phalloides, and of Agaricales, reveals dynamic evolution of toxin genes in an invasive range. The ISME Journal. https://doi.org/10.1038/s41396-023-01432-x
Ramos A., López-Ramírez, F., Flores-Estévez, N., & Miguel, A. (2013). An approach to finding enzymatic restriction patterns for molecular characterization of Amanita muscaria. Journal of Food Agriculture & Environment.
Sa'adah, S., Hidayat, T., & Sudargo, F. (2015). Identifikasi miskonsepsi mahasiswa pendidikan biologi dalam memahami pohon filogenetika.
Saitou N. and Nei M. (1987). The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4:406-425.
Tamura K., Nei M., and Kumar S. (2004). Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Sciences (USA) 101:11030-11035.
Tamura K., Stecher G., and Kumar S. (2021). MEGA 11: Molecular Evolutionary Genetics Analysis Version 11. Molecular Biology and Evolution.
Vargas, N., Gonçalves, S. C., Franco-Molano, A. E., Restrepo, S., & Pringle, A. (2019). In Colombia the Eurasian fungus Amanita muscaria is expanding its range into native, tropical Quercus humboldtii forests. Mycologia.

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Published

2025-05-15

How to Cite

Mutia Andini, T. ., & Achyar, A. . (2025). ANALISIS POHON FILOGENETIK GEN ITS1 Amanita muscaria DARI BERBAGAI NEGARA. Jurnal Biogenerasi, 10(2), 1358–1362. https://doi.org/10.30605/biogenerasi.v10i2.5799