Isolasi dan Uji Patogenesitas Cendawan Endofit Asal Tanaman Pisang Kepok (Musa acuminate balbisiana colla)

Isolation and Inhibition Test of Endophytic Fungi from Kepok Banana (Musa acuminate balbisiana colla)

Authors

  • Eka Lestari Ariyanti Universitas Islam Makassar
  • Rahmat Jahuddin Universitas Islam Makassar
  • Asti Irawanti Azis Universitas Hasanuddin

DOI:

https://doi.org/10.30605/perbal.v13i1.5429

Keywords:

Cendawan endofit, pisang kepok, uji patogenesitas

Abstract

Cendawan endofit dapat digunakan sebagai agens hayati dalam memperbaiki pertumbuhan dan ketahanan tanaman dalam menghadapi serangan penyakit. Penelitian ini bertujuan mengisolasi cendawan endofit asal tanaman pisang dan menguji potensinya sebagai pemacu pertumbuhan tanaman. Berdasarkan hasil isolasi cendawan endofit pada jaringan tanaman pisang kepok diperoleh sebanyak 6 isolat. Hasil identifikasi dan karakterisasi menunjukkan terdapat 4 genus cendawan. Genus cendawan tersebut yaitu Culvularia sp., Fusarium sp., Aspergillus sp., dan Trichoderma sp. Isolat cendawan tersebut diseleksi untuk melihat kemampuan dalam memacu pertumbuhan tanaman melalui uji patogenesitas. Hasil uji patogenesitas menunjukkan cendawan dari genus Trichoderma sp., dan Fusarium sp. memiliki kemampuan terbaik dalam memacu pertumbuhan tanaman. Hal ini mengindikasikan bahwa beberapa cendawan endofit non patogen asal tanaman pisang berpotensi sebagai pemacu pertumbuhan tanaman.

Endophytic fungi can be used as biological agents in improving plant growth and resistance in the face of disease attacks. This study aims to isolate endophytic fungi from banana plants and test their potential as a booster for plant growth. From the results of the isolation of endophytic fungi in the banana pupace plant tissue, 6 isolates were obtained. The results of identification and characterization show that there are 4 genera of fungi. The genus of fungi is Culvularia sp., Fusarium sp., Aspergillus sp., and Trichoderma sp. The fungus isolates were selected to see the ability to spur plant growth through pathogenicity tests. Pathogenicity test results show that fungi from the genus Trichoderma sp., and Fusarium sp. have the best ability to spur plant growth. This indicates that some non-pathogenic endophytic fungi from banana plants have the potential to be a driver of plant growth.

Downloads

Download data is not yet available.

References

Anggraeni, D.N. & Usman, M. (2015). Uji aktivitas dan identifikasi cendawan endofit pada tanah perakaran tanaman pisang (Musa paradisiaca) terhadap cendawan fusarium. BioLink, 1(2): 89-98. DOI: https://doi.org/10.31289/biolink.v1i2.729

Azis, A., I., Rosmana, A., Dewi, V.S. (2013). Pengendalian penyakit hawar daun phytophthora pada bibit kakao dengan Trichoderma asperellum. Jurnal Fitopatologi Indonesia, 9(1): 15-20. DOI: https://doi.org/10.14692/jfi.9.1.15

Badan Pusat Statistik (BPS) dan Direktorat Jenderal Hortikultura. (2020). Produksi Tanaman Buah-Buahan 2020. Badan Pusat Statistik. Jakarta.

Barnett, H.L., & Hunter, B.B. (1998). Illustrated Genera of Imperfect Fungi. Fourth edition. USA: Prentice-Hall.

Foeh, S.C., Temaja, G.R.M, & Khalimi, K. (2019). Potensi bakteri endofit dalam menekan pertumbuhan Phytophthora Palmivora (Butler) secara in vitro. Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology), 18(4): 388-398.

Irawati, A.F., Mutakin, K.H., Suhartono, M.T., Sastro, Y., Sulastri, & Widodo. (2017). Eksplorasi dan pengaruh cendawan endofit yang berasal dari akar tanaman cabai terhadap pertumbuhan benih cabai merah. J.Hort, 27(1): 105-112. DOI: https://doi.org/10.21082/jhort.v27n1.2017.p105-112

Marin-Felix, Y., Hernández-Restrepo, M., & Crous, P. (2020). Multi-locus phylogeny of the genus Curvularia and description of ten new species. Mycological Progress. 19. 559–588. 10.1007/s11557-020-01576-6. DOI: https://doi.org/10.1007/s11557-020-01576-6

Mirsam, H., Kalqutny, S.H., Suriani, Aqil, M., Azrai, M., Pakki, S., Muis, A., Djaenuddin, N., Rauf, A.W., & Muslimin. (2021). Indigenous fungi from corn a potential plant growth promoter and its role in Fusarium verticilliodes suppression on corn. Heliyon, 7(9). DOI: https://doi.org/10.1016/j.heliyon.2021.e07926

Mirsam, H., Suriani, Aqil, M., Azrai, M., Efendi, R., Muliadi, A., Sembiring, H., Azis, A.,I. (2022). Molecular characterization of indigenous microbes and its potential as a biological control agent of Fusarium stem rot disease (Fusarium verticillioides) on maize. Heliyon, 8 (12) :e11960.https://doi.org/10.1016/j.heliyon.2022.e11960. DOI: https://doi.org/10.1016/j.heliyon.2022.e11960

Nashhan, L. (2007). Identifikasi Jamur Patogen Penyebab Penyakit Pascapanen pada Buah Pisang. UNIBRAW. Malang.

Rosmana, A., Junaid, M., Asman, A., Hanum, U. L., Maulydia, L., & Yusuf, K. (2023). Trichoderma asperellum integrated with Fusarium decemcellulare and Lasiodioplodia pseudotheobromae applications increase its colonisation in cacao seedling tissues and suppression of leaf anthracnose disease. IOP Conference Series: Earth and Environmental Science, 1208(1): 012020. DOI: https://doi.org/10.1088/1755-1315/1208/1/012020

Siddiqah, M. (2002). Biodiversitas dan Hubungan Kekerabatan Berdasarkan Karakter Morfologi Berbagai Plasma Nutfah Pisang. IPB. Bogor.

Triwidodo, H., Listihani, & Selangga, D.G.W. (2021). Isolasi cendawan endofit pada tanaman padi serta potensinya sebagai pemacu pertumbuhan tanaman. Agrovigor: Jurnal Agroekoteknologi, 14(2):109-115. DOI: https://doi.org/10.21107/agrovigor.v14i2.10166

Downloads

Published

2025-03-03