ANALISIS HASIL AMPLIFIKASI SAMPEL INDIVIDU LEBAH TANPA SENGAT BERDASARKAN GEN COI DI LAMPUNG TIMUR

Authors

  • Minanti Mayda Ashari
  • Priyambodo Priyambodo
  • Elly Lestari Rustiati
  • Gina Dania Pratami
  • Yuliana Andriyani
  • Eko Agus Srihanto
  • Dian Neli Pratiwi
  • Laila Salwa Azzahra
  • Ersa Imelda Adelia
  • Shifa Sandra
  • Septi Wahyu Lestari
  • Viki Ramadan

DOI:

https://doi.org/10.30605/biogenerasi.v10i2.5540

Abstract

Lebah tanpa sengat masuk ke dalam suku meliponidae dan merupakan lebah yang tidak menggunakan daya sengat sebagai alat pertahanan diri melainkan gigitan dan kerumunan apabila berada dalam bahaya. Indonesia memiliki keragaman jenis lebah tanpa sengat dan telah ditemukan sekitar 52 jenis yang tersebar di Pulau Sumatera, Kalimantan, Jawa, Timor, Sulawesi, Ambon, Maluku dan Irian Jaya. Dari beragam jenis lebah tanpa sengat yang ditemukan di Indonesia menunjukkan bahwa adanya keragaman genetik yang tinggi. Penelitian ini bertujuan untuk melakukan analisis hasil amplifikasi secara kualitatif dari DNA lebah tanpa sengat berdasarkan gen COI. Penelitian dilaksanakan pada rentang bulan Oktober 2024 - Januari 2025. Tahapan dalam penelitian ini meliputi pengambilan sampel, ekstraksi dan amplifikasi DNA.  Hasil ekstraksi dan amplifikasi diuji dengan  mengggunakan elektroforesis. Berdasarkan analisis kualitatif menggunakan gel agarose 1%, keempat sampel lebah tanpa sengat berhasil diekstraksi DNAnya. Ukuran panjang DNA dari masing-masing sampel lebah tanpa sengat diketahui berkisar antara 600-700bp dari hasil uji kualitatif setelah amplifikasi DNA.

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Published

2025-03-02

How to Cite

Ashari, M. M., Priyambodo, P., Rustiati, E. L., Pratami, G. D., Andriyani, Y., Srihanto, E. A., … Ramadan, V. (2025). ANALISIS HASIL AMPLIFIKASI SAMPEL INDIVIDU LEBAH TANPA SENGAT BERDASARKAN GEN COI DI LAMPUNG TIMUR. Jurnal Biogenerasi, 10(2), 1015–1020. https://doi.org/10.30605/biogenerasi.v10i2.5540

Issue

Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)

Section

Articles

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